MLST of Acinetobacter baumannii complex
Genes
The Acinetobacter baumannii complex MLST scheme uses fragments of the following seven house-keeping genes:
Citrate synthase (gltA)
DNA gyrase subunit B (gyrB)
Glucose dehydrogenase B (gdhB)
Homologous recombination factor (recA)
60-kDa chaperonin (cpn60)
Glucose-6-phosphate isomerase (gpi)
RNA polymerase sigma factor(rpoD)
Primers
| Locus | Primer | Sequences | Amplicon size (bp) | Usage |
|---|---|---|---|---|
| gltA | Citrato F1 | AAT TTA CAG TGG CAC ATT AGG TCC C | 722 | amp/seq |
| Citrato R12 | GCA GAG ATA CCA GCA GAG ATA CAC G | amp/seq | ||
| gyrB | APRU F | TGT AAA ACG ACG GCC AGT GCN GGR TCY TTY TCY TGR CA | 909 | amp |
| M13 [-21] | TGT AAA ACG ACG GCC AGT | seq | ||
| UP1E R | CAG GAA ACA GCT ATG ACC AYG SNG GNG GNA ART TYR A | amp | ||
| M13 F | CAG GAA ACA GCT ATG ACC | seq | ||
| gdhB | GDHB 1F | GCT ACT TTT ATG CAA CAG AGC C | 775 | amp |
| GDH SEC F | ACC ACA TGC TTT GTT ATG | seq | ||
| GDHB 775R | GTT GAG TTG GCG TAT GTT GTG C | amp | ||
| GDH SEC R | GTT GGC GTA TGT TGT GC | seq | ||
| recA | RA1 | CCT GAA TCT TCY GGT AAA AC | 425 | amp/seq |
| RA2 | GTT TCT GGG CTG CCA AAC ATT AC | amp/seq | ||
| cpn60 | CPN 3F2 | ACT GTA CTT GCT CAA GC | 479 | amp/seq |
| CPN R2 | TTC AGC GAT GAT AAG AAG TGG | amp/seq | ||
| gpi | GPI F1 | AAT ACC GTG GTG CTA CGG G | 508 | amp/seq |
| GPI R1 | AAC TTG ATT TTC AGG AGC | amp/seq | ||
| rpoD | 70F RPOD | ACG ACT GAC CCG GTA CGC ATG TAY ATG MGN GAR ATG GGN AC NGT | 492 | amp |
| 70FS | ACG ACT GAC CCG GTA CGC ATG TA | seq | ||
| 70R RPOD | ATA GAA ATA ACC AGA CGT AAG TTN GCY TCN ACC ATY TCY TT YTT | amp | ||
| 70RS | ATA GAA ATA ACC AGA CGT AAG TT | seq |