Primers - Lactococcal bacteriophage MLST Databases

Genes

The MLST scheme for the lactococcal phages of the 936-group uses internal fragments of the following five house-keeping genes:

• terL (terminase large subunit)
• mcp (major capsid protein)
• mtp (major tail protein)
• tmp (tail tape-measure protein)
• lys (endolysin)

PCR Amplification

Amplification primers were designed by M. Moisan at the Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Québec, Canada.

The primer pairs we use for the PCR amplification of internal fragments of these genes are:

terL-F   5-GATGATTTTAGGCGGACAAT-3
terL-R   5-AGAACTTATTCTGTAACGCTG-3
mcp-F    5-GCAAAACTTGCTGAAAATGG-3
mcp-R    5-CTCATCTAACAAGGCTTTACG-3
mtp-F    5-TARCTGGTTTAGTATCRGTTGG-3
mtp-R    5-ACTGAWTCTGTTTCTGATTCTT-3
tmp-F    5-AAYTTACAAACGCAGTTGG-3
tmp-R    5-CAAAYGCTTGGTTAGCTTTTA-3
lys-F    5-AACAAACGGAGGATAAAAAAG-3
lys-R    5-AGTTCTGYRATAAARTATGAGC-3

Sequencing

We use the same primer pairs for sequencing.