Multi Locus Sequence Typing of Campylobacter

An MLST system has been developed in the laboratory of Dr. Martin Maiden, Peter Medawar Building for Pathogen Research, University of Oxford, in collaboration with Prof. F. J. Bolton and Prof. A. Fox of the Public Health Laboratory Service.

Preliminary findings and methods have been published.

Dingle, K.E., Colles, F.M., Wareing, D.R.A., Ure, R., Fox, A.J., Bolton, F.J., Bootsma, R.J.L., Willems, R., Urwin, R., Maiden, M.C.J.  Multilocus Sequence Typing System for Campylobacter jejuni.  Journal of Clinical Microbiology (2001) 39 (1), 14-23.

This project is funded by the UK Department for Environment, Food and Rural Affairs (DEFRA).

We are planning to develop an integrated system together with several other interested groups in the UK and elsewhere.

Information

• General information on MLST
• Campylobacter jejuni porA (Product MOMP)

Experimental Conditions

MLST

These protocols are for guidance only and you may need to optimise them for your particular laboratory.

• C. jejuni/coli primers
• C. helveticus primers
• C. insulaenigrae primers
• C. lari primers
• C. upsaliensis primers
• Sequencing protocol (PCR tubes)
• Sequencing protocol (Microtitre plates)

porA (MOMP)

• PCR amplification and sequencing